Research Paper

Enzyme Concentration Coursework

Effect of Substrate Concentration on the Rate of Activity of Catalase ...
Mar 21, 2018 ... Here is an example of A-level biology coursework on the effect of substrate concentration (hydrogen peroxide) on the rate of activity of the ...

Enzyme Concentration Coursework

Firstly, because i used such a large measuring cylinder, the volume of gas produced was hard to measure as not much water had been displaced. Because of this, i decided to grind the yeast into a powder so that the surface area would be more similar in each yeast granule. The reaction is over when you have recorded three volumes of gas that are concordant or very similar.

Do this first to ensure that the mixtures have enough time to reach a constant temperature instead of putting them in for a short time. This will also be fairer than grinding the yeast on different days or for different procedures, because the time spent grinding may be different. Therefore i did a further experiment based on timing only and found that if i measured the volume of gas every 5 seconds i obtained enough measurements.

More importantly, the greater the surface area of the yeast, the more reactions take place because there will be more collisions between the enzyme and substrate molecules. Overall, there is a pattern showing a constant trend in that as the concentration decreases the rate of reaction decreases too, and that the overall volume of gas evolved also decreases. Although i tried to control the temperature in a water bath, and to good effect (a constant external temperature was produced and the heat energy was dissipated), i could not control the amount of heat given off in each reaction.

This can be explained by the collision theory, which states that the time it takes for a reaction to occurand a set volume of gas to be evolvedis shorter for higher concentrations of substrate. This is because there will be more collisions between the enzyme and substrate molecules resulting in more enzyme-substrate complexes. I will work out the gradient of these new results and plot them on a new rate of reaction graph.

. Enzyme activity is generally greatest when substrate concentration is unlimiting. This means the amount of substrate could have been different for each repeat, even though i used the same concentration.

From the graph, i can see that as the concentration of hydrogen peroxide decreased, the volume of oxygen produced decreased as a direct result. Take the conical flask with the 100 hydrogen peroxide out of the water bath and measure exactly 5cm small beaker. This decreased the number of particles that could react with each other, and so the number of collisions that reached the activation energy also decreased. No data is shared with paypal unless you engage with this feature. This is because at higher concentration there are more molecules of substrate, so more collisions take place, resulting in more enzyme-substrate complexes being formed.


Enzyme Concentration (Introduction to Enzymes)


In order to study the effect of increasing the enzyme concentration upon the reaction rate, the substrate must be present in an excess amount; i.e., the reaction ...

Enzyme Concentration Coursework

Problems | Enzymes | InformIT
Apr 27, 2017 ... At an enzyme concentration of 10–6 M, what will be the initial rate of ... [Courtesy of D. J. Kirwan from “Collected Coursework Problems in ...
Enzyme Concentration Coursework The amount of enzyme present in a reaction is measured by the activity it catalyzes. Full table of results, including 10 and 30 concentrations of hydrogen peroxide. For example, if there were more molecules of yeast, Place the conical flask under the gas syringe and place an airtight stopper in the top, with a single tube attached to the gas syringe (as shown in fig. Mar 21, 2018. This would show that the reaction is a first order reaction. Also, the bubble volume is affected by the diameter of the tube and the overall pressure of the water (depth) so i believe that by using the gas syringe, A larger surface area means there are more molecules being exposed to collisions with other molecules. Hopefully, with the new and repeated results, i will be able to analyse my results further and therefore evaluate them with more evidence than i had previously, The reaction is exothermic, meaning heat is produced in the reaction. Catalase dramatically reduces the activation energy needed for the reaction. This decreased the number of particles that could react with each other, and so the number of collisions that reached the activation energy also decreased.
  • Enzymes review (article) | Enzymes | Khan Academy


    In other words, the rate is proportional to the concentration. I did this by only using pipettes, which have a very small apparatus error when compared to beakers. Hubpages and hubbers (authors) may earn revenue on this page based on affiliate relationships and advertisements with partners including amazon, google, and others. In the 60 concentration, the repeats at 5 seconds were 20cm i ground up the yeast to try to make the surface area as similar as possible because surface area is a major factor in my experiment. This results in a bigger proportion of molecules having a kinetic energy greater than that of the activation energy.

    I expect this graph to look similar to the ones i have described above. Carry out each reaction three times to gain an average. Being very careful as not to spill the mixture, take the stopper off the conical flask and lower the beaker into the conical flask using tweezers. However, this does not account for the fact that a concentration of 0 hydrogen peroxide produces 0cm of oxygen. This is used for a registered author who enrolls in the hubpages earnings program and requests to be paid via paypal.

    Since it had a rate of reaction that was so much higher than the other values, i will also repeat the 100 concentration of hydrogen peroxide because i believe this was an anomalous result. Apparatus error was one of the main factors in my experiment which i tried to keep at a minimum. Finally, i also want to determine whether or not half the 100 concentration of hydrogen peroxide (50) will produce half the volume of gas. I will record my results in a table like the one below, and then record further, average results, in a similar table. I also had to change the gas syringe because at first the reaction did not occur because a large volume of gas was leaking from a tear in the tube. I will use the following concentrations of hydrogen peroxide 100, 90, 80, 70, 60 and 50. So, at the 100 concentration of hydrogen peroxide, the oxygen was given off more rapidly because there were more substrate and enzyme molecule reactions. However, some oxygen was displaced in the gas syringe and i had to solve this by subtracting this small amount from the volumes produced in each of the reactions. I solved this by swirling the conical flask constantly throughout the reactions, which seemed to solve the problem, although this meant that the amount of swirling had to be the same in order to ensure a fair test. In my first preliminary experiment, i weighed 1.

    Enzyme concentration: Increasing enzyme concentration will speed up the reaction, as long as there is substrate available to bind to. Once all of the substrate is ...

    Investigate the effect of enzyme concentration on the... | Bartleby

    Free Essay: To investigate the effect of enzyme concentration on the rate of reaction PREDICTION: An enzyme is a biological catalyst. They speed up the rate...
  • Do My Homework Fast
  • Top 5 Essay Writing Services
  • What Is The Best College Essay Editing Service
  • Customized Essay
  • Thesis Software
  • Food And Nutrition Coursework Evaluation
  • Food Technology Coursework Resources
  • Further Maths Coursework
  • Future Coursework Amcas
  • Gce Computing Coursework
  • Career In Creative Writing

    In order to study the effect of increasing the enzyme concentration upon the reaction rate, the substrate must be present in an excess amount i. Overall, i believe my experiment went well and that i gained sufficient results because i repeated each concentration three times and investigated eight concentrations in total. A single end point determination at e would lead to the false conclusion that all three samples had identical enzyme concentration. I did this by only using pipettes, which have a very small apparatus error when compared to beakers. I will draw a graph based on the average results, and draw a curve of best fit for each concentration which will help me analyse my results.

    However, in my next preliminary experiment, i decided this would be unfair in the main procedure Buy now Enzyme Concentration Coursework

    12th Grade Essay

    Between a and b, the curve represents a zero order reaction that is, one in which the rate is constant with time. To find out if the concentrations were accurate as a whole, i worked out the rate of reaction. Each piece of apparatus has an apparatus error with an upper and lower limit. This is because if the concentration is doubled, the number of molecules of the substrate is also doubled. The accuracy of the results showed that this factor did not distort the results too much, and so a similar amount of substrate molecules were present in each reaction.

    The hydrogen peroxide is inserted into a 5cm and then tipped over to spill the contents and start the reaction. This is because at higher concentrations, there are more substrate molecules than in lower concentrations Enzyme Concentration Coursework Buy now

    Phd Thesis Writing Services In Bangalore

    B is a straight line representing a zero order reaction which permits accurate determination of enzyme activity for part or all of the reaction time. The initial rate of reaction was fastest for the 100 concentration of hydrogen peroxide and gradually decreased with each successive concentration (90, 80, etc. Carry out each reaction three times to gain an average. The hydrogen peroxide is injected into the test tube, and the volume of oxygen gas is recorded (by the amount of water displaced), determining the rate of reaction. No data is shared unless you engage with this feature.

    For example, the balance had an apparatus error of 0. Because of this, it is very important to ensure a constant temperature is maintained Buy Enzyme Concentration Coursework at a discount

    Alexander Pope Essay Man Full Text

    Figure 7 illustrates three types of reactions which might be encountered in enzyme assays and shows the problems which might be enountered if only single measurements are made. I would expect this graph to be linear as this would show that as the concentration increases, the time taken for a set volume of gas would decrease. A larger surface area means there are more molecules being exposed to collisions with other molecules, with sufficient energy to cause a reaction. I will work out the rate of reaction from the results gained in the first 5 seconds as this will be the point where the greatest volume of gas evolves. In figure 5, activity is directly proportional to concentration in the area ab, but not in bc Buy Online Enzyme Concentration Coursework

    Against Nature Essay

    This is because fewer overall collisions took place, and so a reduced number of collisions reached the activation energy. Overall, i believe my experiment went well and that i gained sufficient results because i repeated each concentration three times and investigated eight concentrations in total. For example, the 100 concentration of hydrogen peroxide evolved a final average volume of gas of 77cm of oxygen while the 90 concentration evolved a final average volume of 73. Apparatus error was one of the main factors in my experiment which i tried to keep at a minimum. This enabled me to work out an average by adding up three repeat values and dividing by 3.

    This is because at higher concentration there are more molecules of substrate, so more collisions take place, resulting in more enzyme-substrate complexes being formed Buy Enzyme Concentration Coursework Online at a discount

    Americans Essay

    This would show that the reaction is a first order reaction. This will vary as a direct result of the different concentrations of hydrogen peroxide. More importantly, the greater the surface area of the yeast, the more reactions take place because there will be more collisions between the enzyme and substrate molecules. Because a pipette is a very accurate way of measuring volumes, i believe that this will be the best method to make the concentrations. So, for example, the 40 concentration should have a curve gradient near to the value of 3.

    As substrate is used up, the enzymes active sites are no longer saturated, substrate concentration becomes rate limiting, and the reaction becomes first order between b and c Enzyme Concentration Coursework For Sale

    Anger Deadly Enigma Essay Sin Sometimes

    Here are the proposed solutions to the problems i identified. However, this presents a problem because this either suggests that the concentration of 100 is not accurate and is an anomaly, or that the line of best fit should actually be a curve of best fit. Therefore it is true to say that in theory, rate concentration. Comscore is a media measurement and analytics company providing marketing data and analytics to enterprises, media and advertising agencies, and publishers. A larger surface area means there are more molecules being exposed to collisions with other molecules, with sufficient energy to cause a reaction.

    Hopefully this will mean that each yeast granule will have the same (or a very similar) surface area For Sale Enzyme Concentration Coursework

    Alice Walker Essay Beauty When The Other Dancer Is The Self

    Since it had a rate of reaction that was so much higher than the other values, i will also repeat the 100 concentration of hydrogen peroxide because i believe this was an anomalous result. The reaction is over when you have recorded three volumes of gas that are concordant or very similar. The raw results can be seen in the appendix. Overall, i believe my experiment went well and that i gained sufficient results because i repeated each concentration three times and investigated eight concentrations in total. However, in my next preliminary experiment, i decided this would be unfair in the main procedure.

    Represent the results in a graph in order to work out the gradient and make a conclusion based on the evidence you have obtained Sale Enzyme Concentration Coursework

    MENU

    Home

    Business plan

    Critical

    Rewiew

    Research

    Paper

    Literature

    Letter

    Coursework

    Case study

    Bibliography

    An Essay On The Best Day Of My Life

    University Of Houston Creative Writing Faculty

    How To End A Creative Writing Story

    An Essay On Respecting Elders

    A Raisin In The Sun Dream Essay

    An Essay On A Heroic Act

    What Is Creative Writing Ppt

    How To Write A Persuasive Speech About Abortion

    Free Writing Checker Software

    A Persuasive Essay On Uniforms In School

    Creative Copywriting Portfolio

    Amusement Park Description Essay

    America And The Cold War Essay

    A Day In My Life Essay

    U Of T Creative Writing Undergraduate

    Research Paper
    sitemap

    SPONSOR