In order to study the effect of increasing the enzyme concentration upon the reaction rate, the substrate must be present in an excess amount i. Overall, i believe my experiment went well and that i gained sufficient results because i repeated each concentration three times and investigated eight concentrations in total. A single end point determination at e would lead to the false conclusion that all three samples had identical enzyme concentration. I did this by only using pipettes, which have a very small apparatus error when compared to beakers. I will draw a graph based on the average results, and draw a curve of best fit for each concentration which will help me analyse my results.
However, in my next preliminary experiment, i decided this would be unfair in the main procedure Buy now Enzyme Concentration Coursework
Between a and b, the curve represents a zero order reaction that is, one in which the rate is constant with time. To find out if the concentrations were accurate as a whole, i worked out the rate of reaction. Each piece of apparatus has an apparatus error with an upper and lower limit. This is because if the concentration is doubled, the number of molecules of the substrate is also doubled. The accuracy of the results showed that this factor did not distort the results too much, and so a similar amount of substrate molecules were present in each reaction.
The hydrogen peroxide is inserted into a 5cm and then tipped over to spill the contents and start the reaction. This is because at higher concentrations, there are more substrate molecules than in lower concentrations Enzyme Concentration Coursework Buy now
B is a straight line representing a zero order reaction which permits accurate determination of enzyme activity for part or all of the reaction time. The initial rate of reaction was fastest for the 100 concentration of hydrogen peroxide and gradually decreased with each successive concentration (90, 80, etc. Carry out each reaction three times to gain an average. The hydrogen peroxide is injected into the test tube, and the volume of oxygen gas is recorded (by the amount of water displaced), determining the rate of reaction. No data is shared unless you engage with this feature.
For example, the balance had an apparatus error of 0. Because of this, it is very important to ensure a constant temperature is maintained Buy Enzyme Concentration Coursework at a discount
Figure 7 illustrates three types of reactions which might be encountered in enzyme assays and shows the problems which might be enountered if only single measurements are made. I would expect this graph to be linear as this would show that as the concentration increases, the time taken for a set volume of gas would decrease. A larger surface area means there are more molecules being exposed to collisions with other molecules, with sufficient energy to cause a reaction. I will work out the rate of reaction from the results gained in the first 5 seconds as this will be the point where the greatest volume of gas evolves. In figure 5, activity is directly proportional to concentration in the area ab, but not in bc Buy Online Enzyme Concentration Coursework
This is because fewer overall collisions took place, and so a reduced number of collisions reached the activation energy. Overall, i believe my experiment went well and that i gained sufficient results because i repeated each concentration three times and investigated eight concentrations in total. For example, the 100 concentration of hydrogen peroxide evolved a final average volume of gas of 77cm of oxygen while the 90 concentration evolved a final average volume of 73. Apparatus error was one of the main factors in my experiment which i tried to keep at a minimum. This enabled me to work out an average by adding up three repeat values and dividing by 3.
This is because at higher concentration there are more molecules of substrate, so more collisions take place, resulting in more enzyme-substrate complexes being formed Buy Enzyme Concentration Coursework Online at a discount
This would show that the reaction is a first order reaction. This will vary as a direct result of the different concentrations of hydrogen peroxide. More importantly, the greater the surface area of the yeast, the more reactions take place because there will be more collisions between the enzyme and substrate molecules. Because a pipette is a very accurate way of measuring volumes, i believe that this will be the best method to make the concentrations. So, for example, the 40 concentration should have a curve gradient near to the value of 3.
As substrate is used up, the enzymes active sites are no longer saturated, substrate concentration becomes rate limiting, and the reaction becomes first order between b and c Enzyme Concentration Coursework For Sale
Here are the proposed solutions to the problems i identified. However, this presents a problem because this either suggests that the concentration of 100 is not accurate and is an anomaly, or that the line of best fit should actually be a curve of best fit. Therefore it is true to say that in theory, rate concentration. Comscore is a media measurement and analytics company providing marketing data and analytics to enterprises, media and advertising agencies, and publishers. A larger surface area means there are more molecules being exposed to collisions with other molecules, with sufficient energy to cause a reaction.
Hopefully this will mean that each yeast granule will have the same (or a very similar) surface area For Sale Enzyme Concentration Coursework
Since it had a rate of reaction that was so much higher than the other values, i will also repeat the 100 concentration of hydrogen peroxide because i believe this was an anomalous result. The reaction is over when you have recorded three volumes of gas that are concordant or very similar. The raw results can be seen in the appendix. Overall, i believe my experiment went well and that i gained sufficient results because i repeated each concentration three times and investigated eight concentrations in total. However, in my next preliminary experiment, i decided this would be unfair in the main procedure.
Represent the results in a graph in order to work out the gradient and make a conclusion based on the evidence you have obtained Sale Enzyme Concentration Coursework